Transcriptomic Response of L. monocytogenes to Co-Culture with S. cerevisiae

The aim of the present study was to assess transcriptomic response L. monocytogenes during co-culture with three S. cerevisiae strains. For this purpose, BHI broth inoculated 7 log CFU?mL?1 serotype 4b strain LQC 15257, isolated from a strawberry sample and 4 strains Y32, Y34 Y37, spontaneous olive fermentation. Sampling took place after 24 48 h incubation at 5 20 °C. RNA extracted, stabilized transcription virulence associated genes prfA, sigB, hly, plcA, plcB, inlA, inlB, inlC inlJ, assessed by RT-qPCR. Co-culture yeast mostly affected sigB upregulation which growth °C for h, reached 10.13 9.76 log2(fold change), respectively. Similarly, effect that time had on relative under dependent co-cultivating strain. On other hand, less pronounced when between In case, temperature seemed have an important since, in 79.2% samples analyzed, evident, irrespective presence. These results highlight complex trophic relationships take co-existence cerevisiae.